chemiluminescence ecl kit (Bio-Rad)
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chemiluminescence ecl kit
Chemiluminescence Ecl Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 15497 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/clarity+western+ecl+substrate+kit/Clarity+Western+ECL+Substrate/pmc13181336-273-6-13
Average 99 stars, based on 15497 article reviews
Chemiluminescence Ecl Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 15497 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/clarity+western+ecl+substrate+kit/Clarity+Western+ECL+Substrate/pmc13181336-273-6-13
Average 99 stars, based on 15497 article reviews
chemiluminescence ecl kit - by Bioz Stars,
2026-09
99/100 stars
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Western Blot:Article Title: DGAT1 Drives Racially Divergent Fibroblast Activation via ERK1/2-Dependent Tumorigenic Signaling in Prostate Cancer Article Snippet: .. C R C - 2 5 - 0 7 0 1 / 3 7 9 3 7 3 3 / c r c - 2 5 - 0 7 0 1 . p d f b y g u e s t o n 1 5 M a y 2 0 2 6 temperature with appropriate HRP-conjugated secondary antibodies (Cell Signaling Technologies Inc. at 1:5000 dilution), followed by exposure to Article Title: Pharmacological Rescue of Follicle-Stimulating Hormone Receptor Mutants. In vitro and In silico Studies☨. Article Snippet: Equal protein loading was confirmed in a 4 membrane reprobed with primary polyclonal antibody against total ERK1/2 (1:10,000) 5 (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA) (RRID: AB_2141292 hwsorA, 6 http://antibodyregistry.org/AB_2141292). .. Signal development was performed using the 7 Article Title: Inflammation-Linked Muscle Atrophy in Limb Girdle Muscular Dystrophy R1 (LGMDR1): Insights into Disease Mechanisms. Article Snippet: After this, membrane was blocked using 5% skimmed milk in 1× TBST for 1 h (For phospho antibody, membrane was blocked using 5% BSA in 1× TBST for 1 h) and then incubated with primary antibodies: Anti-MuRF1 antibody (Santa Cruz Biotechnology, Dallas, TX, USA; #398608); Anti-Fbx32 antibody (Abcam, Cambridge, UK; #168372); anti-NFκB antibody (Cell Signaling Technology, Danvers, MA, USA; #4764); antiphospho-NFκB (Ser 536) antibody (Cell Signaling Technology, Danvers, MA, USA; #3033); anti-FOXO1 antibody (Cell Signaling Technology, Danvers, MA, USA; #2880); anti-IκBα antibody (Cell Signaling Technology, Danvers, MA, USA; #4814), anti-phospho- IκBα (Ser 32/36) antibody (Cell Signaling Technology, Danvers, MA, USA; #9246); anti-phosphoFOXO1 (Ser 256) antibody (Cell Signaling Technology, Danvers, MA, USA; #9461); anti-AKT antibody (Cell Signaling Technology, Danvers, MA, USA; #9272); anti-phospho-AKT (Ser 473) antibody (Cell Signaling Technology, Danvers, MA, USA; #9271) at 4 ◦C, overnight. .. The next day, the membrane was incubated with an appropriate secondary antibody for 1 h at room temperature and developed with chemiluminescence substrate (Biorad Article Title: Pharmacological Rescue of Follicle-Stimulating Hormone Receptor Mutants. In vitro and In silico Studies☨. Article Snippet: .. 17 Signal development was performed using the Article Title: Mechanistic Insights into Piperine-Driven Oxidative Stress, Autophagy Activation and Anti-Migration Effects in Caco-2 Cells. Article Snippet: Membranes were washed and incubated with appropriate secondary antibodies (Goat anti-Rabbit and Goat anti-Mouse; Bio-Rad; dilution 1:5000) for 1 h at room temperature. .. Protein bands were visualized using the Article Title: Nuclear proteome reprogramming and acquired thermotolerance in chickpea exposed to escalating high-temperature stress. Article Snippet: The protein samples were resolved through 12.5% SDS-PAGE, electrotransferred onto nitrocellulose membrane, and incubated with anti-histone H3 (Abcam Limited, U.K.), anti-CPN60 (chaperonin 60, Agrisera) and anti-RbCL (RuBisCO Large subunit, Agrisera) antibodies followed by HRP-conjugated secondary antibody (Abcam Limited, U.K.) incubation. .. The chemiluminescence was detected on X-ray films (Carestream) using an enhanced Article Title: Proteomic profiling of ANO10-deficient SH-SY5Y cells reveals candidate pathways relevant to SCAR10. Article Snippet: .. Proteins were detected after overnight incubation with primary antibodies, followed by a 1-hour incubation with secondary antibodies (see Supplementary Material 9, Table S5), using the Article Title: Potassium suppresses allosteric activation of ZAP-70-dependent T Cell Receptor signaling. Article Snippet: .. The blot was washed three times with 1× TBST and two times with 1XTBS before developing with the Membrane:Article Title: Inflammation-Linked Muscle Atrophy in Limb Girdle Muscular Dystrophy R1 (LGMDR1): Insights into Disease Mechanisms. Article Snippet: After this, membrane was blocked using 5% skimmed milk in 1× TBST for 1 h (For phospho antibody, membrane was blocked using 5% BSA in 1× TBST for 1 h) and then incubated with primary antibodies: Anti-MuRF1 antibody (Santa Cruz Biotechnology, Dallas, TX, USA; #398608); Anti-Fbx32 antibody (Abcam, Cambridge, UK; #168372); anti-NFκB antibody (Cell Signaling Technology, Danvers, MA, USA; #4764); antiphospho-NFκB (Ser 536) antibody (Cell Signaling Technology, Danvers, MA, USA; #3033); anti-FOXO1 antibody (Cell Signaling Technology, Danvers, MA, USA; #2880); anti-IκBα antibody (Cell Signaling Technology, Danvers, MA, USA; #4814), anti-phospho- IκBα (Ser 32/36) antibody (Cell Signaling Technology, Danvers, MA, USA; #9246); anti-phosphoFOXO1 (Ser 256) antibody (Cell Signaling Technology, Danvers, MA, USA; #9461); anti-AKT antibody (Cell Signaling Technology, Danvers, MA, USA; #9272); anti-phospho-AKT (Ser 473) antibody (Cell Signaling Technology, Danvers, MA, USA; #9271) at 4 ◦C, overnight. .. The next day, the membrane was incubated with an appropriate secondary antibody for 1 h at room temperature and developed with chemiluminescence substrate (Biorad Incubation:Article Title: Inflammation-Linked Muscle Atrophy in Limb Girdle Muscular Dystrophy R1 (LGMDR1): Insights into Disease Mechanisms. Article Snippet: After this, membrane was blocked using 5% skimmed milk in 1× TBST for 1 h (For phospho antibody, membrane was blocked using 5% BSA in 1× TBST for 1 h) and then incubated with primary antibodies: Anti-MuRF1 antibody (Santa Cruz Biotechnology, Dallas, TX, USA; #398608); Anti-Fbx32 antibody (Abcam, Cambridge, UK; #168372); anti-NFκB antibody (Cell Signaling Technology, Danvers, MA, USA; #4764); antiphospho-NFκB (Ser 536) antibody (Cell Signaling Technology, Danvers, MA, USA; #3033); anti-FOXO1 antibody (Cell Signaling Technology, Danvers, MA, USA; #2880); anti-IκBα antibody (Cell Signaling Technology, Danvers, MA, USA; #4814), anti-phospho- IκBα (Ser 32/36) antibody (Cell Signaling Technology, Danvers, MA, USA; #9246); anti-phosphoFOXO1 (Ser 256) antibody (Cell Signaling Technology, Danvers, MA, USA; #9461); anti-AKT antibody (Cell Signaling Technology, Danvers, MA, USA; #9272); anti-phospho-AKT (Ser 473) antibody (Cell Signaling Technology, Danvers, MA, USA; #9271) at 4 ◦C, overnight. .. The next day, the membrane was incubated with an appropriate secondary antibody for 1 h at room temperature and developed with chemiluminescence substrate (Biorad Article Title: Proteomic profiling of ANO10-deficient SH-SY5Y cells reveals candidate pathways relevant to SCAR10. Article Snippet: .. Proteins were detected after overnight incubation with primary antibodies, followed by a 1-hour incubation with secondary antibodies (see Supplementary Material 9, Table S5), using the Expressing:Article Title: Mechanistic Insights into Piperine-Driven Oxidative Stress, Autophagy Activation and Anti-Migration Effects in Caco-2 Cells. Article Snippet: Membranes were washed and incubated with appropriate secondary antibodies (Goat anti-Rabbit and Goat anti-Mouse; Bio-Rad; dilution 1:5000) for 1 h at room temperature. .. Protein bands were visualized using the |